Polymorphisms of genes encoding tumor necrosis factor-alpha, interleukin-10, cluster of differentiation-14 and interleukin-1ra in critically ill patients

Abstract 

Purpose

The aim of the study was to determine whether distributions of tumor necrosis factor (TNF)-α₃₀₈, interleukin (IL)-10₁₀₈₂, CD14₁₅₉, and IL-1ra gene intron 2 genotypes in critically ill patients are associated with outcome, underlying cause of sepsis, and type of microorganism.

Materials and Methods

Blood samples from 106 critically ill white patients were genotyped by method based on polymerase chain reaction for TNF-α₃₀₈, IL-10₁₀₈₂, CD14₁₅₉, and IL-1ra gene intron 2.

Results

All patients with TNF-α₃₀₈AA genotype survived; relative risk (RR) of death in patients with AG was 3.250 and with GG, 1.923 (P < .01). In patients with Gram-positive sepsis, IL-10₁₀₈₂AA and then AG genotypes were the most frequent ones (odds ratio [OR], 18.67 and 7.20, respectively; P < .01). When comparing IL-10₁₀₈₂AA with AG, RR of pancreatitis was 1.80 and OR was 3.40. When AA and GG were compared, RR was 7.33 and OR was 20.00. In patients with GG, RR of peritonitis was 4.07 and OR was 5.88 (P < .01). In patients with Gram-positive sepsis, CD14₁₅₉CT was the most frequent one with OR of 5.25. Distribution of 6 IL-1ra gene intron 2 genotypes showed no significant association.

Conclusions

Distribution of TNF-α₃₀₈ genotypes is associated with outcome, IL-10₁₀₈₂ with type of microorganism and underlying cause of sepsis, and CD14₁₅₉ with type of microorganism.

Keywords: Sepsis, Trauma, Gene polymorphisms